منابع مشابه
Development of a Nano-ELISA system for the rapid and sensitive detection of H9N2 avian influenza
Influenza is one of the most important viral diseases that is common among the birds and the mammalsand is caused by specific viruses that belong to the Orthomyxoviridae family. Migratory aquatic birds arethe reservoir of the disease and there is a likelihood of the disease in any region. There are differentmethods for detecting the avian influenza, but by the point of detection rates, the ELIS...
متن کاملSensitive ELISA for measuring elastin breakdown products in plasma.
SD Kucich et al. (1) described an enzyme-linked immunosorbent assay (EUSA) for soluble degradation products of elastin (elastin-derived peptides, EDP) in plasma. They reported a nonspecific loss of measurable EDP owing to coacervation of the elastin-a physical phenomenon in which certain proteins exhibit reversible aggregation on heating or increasing the ionic strength (2). We modified an ELIS...
متن کاملSensitive enzyme linked immunosorbent assay (ELISA) for xanthine oxidase.
The enzyme, xanthine oxidase, has been implicated as a major source of destructive free radicals in ischemia-reperfusion injury associated with a range of pathological states [l]. Enzymic activity has been detected in many mammalian tissues [4] by using, variously, spectrophotometric [51, radiometric[6] and fluorimetric [71 assays. Apart from bovine milk fat globule membrane, which has long pro...
متن کاملSensitive ELISA method for quantitating antibodies to specific protein epitopes.
The detection and quantitation of antibodies to specific peptide epitopes can be a useful technique in many areas of biological research. One application of this technique is in the production of anti-peptide antibodies, a popular method for producing antibodies to single epitopes of proteins, cloned but unpurified proteins or difficult-to-purify proteins. In this report, we describe a simple, ...
متن کاملRapid and sensitive method for erythropoietin determination in serum.
An enzyme-linked immunosorbent assay (ELISA) method for erythropoietin determination has been established by using two kinds of monoclonal antibodies with specific affinities to erythropoietin. Besides being rapid (2.5 h) and sensitive (detection limit 0.3 int. unit/L), the present method gives accurate results and is easy to perform. The method may be clinically applicable for discriminative d...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2000
ISSN: 0736-6205,1940-9818
DOI: 10.2144/00292bm03